Androsterone

Butenandt isolated androsterone, a hormone produced by the testicle that stimulates male development. To do this, he distilled over 17,000 of male urine, from which they got 50 milligrams (0.77 gr) of crystalline androsterone. The isolation and analysis provided insights into male sex hormones.
 Adolf Friedrich Butenandt Germany 1931 Androsterone

Butenandt isolated androsterone, a hormone produced by the testicle that stimulates male development. To do this, he distilled over 17,000 of male urine, from which they got 50 milligrams (0.77 gr) of crystalline androsterone. The isolation and analysis provided insights into male sex hormones.